The Nelson lab uses phage display to construct and use libraries of synthetic antibodies for both the understanding of biological mechanisms underlying cancers as well as their treatment.
Extensive Research Description
An essential component in understanding the function of a gene and its corresponding protein product depends on the existence of high quality antibodies that allow elucidation of a variety of basic questions. This can include cellular and tissue localization and identification of interacting partners through to more detailed analysis provided by an antibody capable of modulating protein function. In this manner, antibodies are key reagents to determine how genes function at the protein level during normal state and, importantly, their role in the initiation and progression of disease.
The Nelson lab uses phage display to construct and use libraries of synthetic antibodies for both the understanding of biological mechanisms underlying cancers as well as their treatment. Phage display allows for the rapid selection of highly specific antibodies from libraries of fully humanized synthetic antibody fragments (Fabs) containing billions of unique clones. Since selections are performed in vitro, exquisite control over conditions can lead to isolation of antibodies not possible by traditional methods. This includes conformation specific antibodies, antibodies specific to each homologous proteins, or transmembrane proteins. Furthermore, since phage display provides a direct link to Fab sequence, affinity maturation, whereby attributes such as affinity and specificity can be modified for use, is possible.
Structural basis for KIT receptor tyrosine kinase inhibition by antibodies targeting the D4 membrane-proximal region.
Proc Natl Acad Sci U S A. 2013 Oct 29;110(44):17832-7. doi: 10.1073/pnas.1317118110. Epub 2013 Oct 14.
- Complete list of publications http://www.ncbi.nlm.nih.gov/sites/myncbi/1-CmepLO6ijkC/bibliography/49294313/public/?sort=date&direction=ascending